Journal: DARU Journal of Pharmaceutical Sciences
Article Title: 14,15-Epoxyeicosatrienoic acid induces the proliferation and anti- apoptosis of human carcinoma cell
doi:
Figure Lengend Snippet: Activation of PPARγ by 100 nM 14,15-EET in carcinoma cells. Cells were seeded in 24 well plates and separated into six groups. The third and sixth groups were co-transfected using 0.4 μg PPRE-tk-Luc, 0.4 μg pCMX3/PPARγ and 0.1 μg control plasmid (phRL-TK). Other groups were transfected by using 0.4 μg PPRE-tk-Luc and 0.1 μg control plasmid (phRL-TK). The first group was incubated using DMSO as negative control. The fifth and sixth groups were administrated with Ciglitazone (20 μM) as positive control. The third and fourth groups were then incubated with 14,15-EET (100 nM) in the absence or presence of GW9662. Data points and error bars represent means±S.E. Significant difference is denoted as asterisk (*) between control and treatments.
Article Snippet: 2-Chloro-5-nitro-N-phenyl-benzamide (GW9662) was purchased from Sigma (St. Louis, USA).
Techniques: Activation Assay, Transfection, Plasmid Preparation, Incubation, Negative Control, Positive Control